Organized at Camp Dick Robinson, Ky., and mustered into the United States service on October 8, 1861. During its first six months of active service, it was stationed at various places in Kentucky, having been assigned to Hascall's Brigade, of Wood's Division. It embarked for Nashville on the 18th of March, 1862, marching thence with Buell's Army to the battle-field of Shiloh, where it arrived at the close of the fighting. After participating in the Siege of Corinth, it marched with Buell through Northern Alabama, Tennessee and Kentucky to Louisville; thence on the Perryville campaign, and then to Nashville, where it arrived in December, 1862. At the battle of Stone's River— still in Wood's Division— it was actively engaged, with a loss of 14 killed, 85 wounded, and 34 missing, Colonel McKee being among the killed. Remaining at Murfreesboro, Tenn., until June, 1863, it moved southward with the army, and fought at Chickamauga, it being then in Harker's (3d) Brigade, Wood's (1st) Division, Crittenden's Corps; its loss in that battle was 13 killed, 78 wounded, and 22 missing. In October, 1863, the regiment was placed in Harker's (3d) Brigade, Sheridan's (2d) Division, Fourth Corps, in which command it fought at Missionary Ridge; loss, 4 killed, and 54 wounded. This division was commanded by General Newton during the Atlanta campaign; the brigade remained under command of General Harker, who was killed while leading the assault on Kenesaw. After the fall of Atlanta, the regiment moved to Nashville, where it was mustered out in October, 1864, its term of service having expired.